CUNE
Species and Locus
Point mutation
5'-NNNNN-3'
3'-NNNNN-5'
Genomic region
Genomic sequence
5'-......-3'
3'-......-5'
Amino acid
Not in a translated region.

Base editors

HDR

Targets Predicted efficiency

Target 1 (2 bases from PAM) (+ strand)

gRNA:

5'-TTTTCAGTTTATATCAGCTTAGG-3'

ssODN:

5'-TAAAAATGAGCAAACTTTTAAATTAGGATTCAAGCCAGAACGTTTTTCAGTTTATATCAGNTTAGGGGCTATGTGCATGTAAAACAGATTAGAAAAGTTTCATGGCGAAGTAACTGAAGAT-3'
Low

Target 2 (3 bases from PAM) (+ strand)

gRNA:

5'-TTTCAGTTTATATCAGCTTAGGG-3'

ssODN:

5'-TAAAAATGAGCAAACTTTTAAATTAGGATTCAAGCCAGAACGTTTTTCAGTTTATATCAGNTTAGGGGCTATGTGCATGTAAAACAGATTAGAAAAGTTTCATGGCGAAGTAACTGAAGAT-3'
Low

Target 3 (4 bases from PAM) (+ strand)

gRNA:

5'-TTCAGTTTATATCAGCTTAGGGG-3'

ssODN:

5'-TAAAAATGAGCAAACTTTTAAATTAGGATTCAAGCCAGAACGTTTTTCAGTTTATATCAGNTTAGGGGCTATGTGCATGTAAAACAGATTAGAAAAGTTTCATGGCGAAGTAACTGAAGAT-3'
Low
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