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CUNE
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Species and Locus
Genome
Chromosome
Location
Point mutation
Mutation (5' - 3') (+ strand)
5'-NNNNN-3'
3'-NNNNN-5'
Genomic region
Genomic sequence
5'-...
C
T
G
A
G
G
C
T
T
C
C
T
G
C
T
C
C
T
C
A
A
C
G
G
A
G
A
C
A
C
C
T
T
T
C
C
A
A
T
T
A
A
A
A
C
A
G
T
A
G
A
C
A
T
G
G
G
A
A
C
T
T
T
A
G
T
G
A
T
G
T
...-3'
3'-...
G
A
C
T
C
C
G
A
A
G
G
A
C
G
A
G
G
A
G
T
T
G
C
C
T
C
T
G
T
G
G
A
A
A
G
G
T
T
A
A
T
T
T
T
G
T
C
A
T
C
T
G
T
A
C
C
C
T
T
G
A
A
A
T
C
A
C
T
A
C
A
...-5'
Amino acid
4930412O13Rik-201
Codon:
T
C
C
(105 out of 106)
Serine (Ser)
9230102O04Rik-201
Codon:
G
G
A
(7 out of 82)
Glycine (Gly)
Base editors
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HDR
Targets
Predicted efficiency
Target 1 (3 bases from PAM) (− strand)
gRNA:
5'-GTCTACTGTTTTAATTGGAAAGG-3'
ssODN:
5'-TGACTTTTAGCAACCTTCTTTCTTTACATCACTAAAGTTCCCATGTCTACTGTTTTAATTNGAAAGGTGTCTCCGTTGAGGAGCAGGAAGCCTCAGCCAGCATGGCCCCGCTGCTTGCTTC-3'
High
Target 2 (17 bases from PAM) (− strand)
gRNA:
5'-TTGGAAAGGTGTCTCCGTTGAGG-3'
ssODN:
5'-TGACTTTTAGCAACCTTCTTTCTTTACATCACTAAAGTTCCCATGTCTACTGTTTTAATTNGAAAGGTGTCTCCGTTGAGGAGCAGGAAGCCTCAGCCAGCATGGCCCCGCTGCTTGCTTC-3'
High
Target 3 (17 bases from PAM) (+ strand)
gRNA:
5'-TCCAATTAAAACAGTAGACATGG-3'
ssODN:
5'-GAAGCAAGCAGCGGGGCCATGCTGGCTGAGGCTTCCTGCTCCTCAACGGAGACACCTTTCNAATTAAAACAGTAGACATGGGAACTTTAGTGATGTAAAGAAAGAAGGTTGCTAAAAGTCA-3'
Low
Target 4 (18 bases from PAM) (+ strand)
gRNA:
5'-CCAATTAAAACAGTAGACATGGG-3'
ssODN:
5'-GAAGCAAGCAGCGGGGCCATGCTGGCTGAGGCTTCCTGCTCCTCAACGGAGACACCTTTCNAATTAAAACAGTAGACATGGGAACTTTAGTGATGTAAAGAAAGAAGGTTGCTAAAAGTCA-3'
Low
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