CUNE
Species and Locus
Point mutation
5'-NNNNN-3'
3'-NNNNN-5'
Genomic region
Genomic sequence
5'-...CTGAGGCTTCCTGCTCCTCAACGGAGACACCTTTCCAATTAAAACAGTAGACATGGGAACTTTAGTGATGT...-3'
3'-...GACTCCGAAGGACGAGGAGTTGCCTCTGTGGAAAGGTTAATTTTGTCATCTGTACCCTTGAAATCACTACA...-5'
Amino acid

4930412O13Rik-201

Codon: TCC (105 out of 106)
Serine (Ser)

9230102O04Rik-201

Codon: GGA (7 out of 82)
Glycine (Gly)

Base editors

HDR

Targets Predicted efficiency

Target 1 (3 bases from PAM) (− strand)

gRNA:

5'-GTCTACTGTTTTAATTGGAAAGG-3'

ssODN:

5'-TGACTTTTAGCAACCTTCTTTCTTTACATCACTAAAGTTCCCATGTCTACTGTTTTAATTNGAAAGGTGTCTCCGTTGAGGAGCAGGAAGCCTCAGCCAGCATGGCCCCGCTGCTTGCTTC-3'
High

Target 2 (17 bases from PAM) (− strand)

gRNA:

5'-TTGGAAAGGTGTCTCCGTTGAGG-3'

ssODN:

5'-TGACTTTTAGCAACCTTCTTTCTTTACATCACTAAAGTTCCCATGTCTACTGTTTTAATTNGAAAGGTGTCTCCGTTGAGGAGCAGGAAGCCTCAGCCAGCATGGCCCCGCTGCTTGCTTC-3'
High

Target 3 (17 bases from PAM) (+ strand)

gRNA:

5'-TCCAATTAAAACAGTAGACATGG-3'

ssODN:

5'-GAAGCAAGCAGCGGGGCCATGCTGGCTGAGGCTTCCTGCTCCTCAACGGAGACACCTTTCNAATTAAAACAGTAGACATGGGAACTTTAGTGATGTAAAGAAAGAAGGTTGCTAAAAGTCA-3'
Low

Target 4 (18 bases from PAM) (+ strand)

gRNA:

5'-CCAATTAAAACAGTAGACATGGG-3'

ssODN:

5'-GAAGCAAGCAGCGGGGCCATGCTGGCTGAGGCTTCCTGCTCCTCAACGGAGACACCTTTCNAATTAAAACAGTAGACATGGGAACTTTAGTGATGTAAAGAAAGAAGGTTGCTAAAAGTCA-3'
Low