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CUNE
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Species and Locus
Genome
Chromosome
Location
Point mutation
Mutation (5' - 3') (+ strand)
5'-NNNNN-3'
3'-NNNNN-5'
Genomic region
Genomic sequence
5'-...
G
G
A
T
T
C
A
A
G
C
C
A
G
A
A
C
G
T
T
T
T
T
C
A
G
T
T
T
A
T
A
T
C
A
G
C
T
T
A
G
G
G
G
C
T
A
T
G
T
G
C
A
T
G
T
A
A
A
A
C
A
G
A
T
T
A
G
A
A
A
A
...-3'
3'-...
C
C
T
A
A
G
T
T
C
G
G
T
C
T
T
G
C
A
A
A
A
A
G
T
C
A
A
A
T
A
T
A
G
T
C
G
A
A
T
C
C
C
C
G
A
T
A
C
A
C
G
T
A
C
A
T
T
T
T
G
T
C
T
A
A
T
C
T
T
T
T
...-5'
Amino acid
Not in a translated region.
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HDR
Targets
Predicted efficiency
Target 1 (2 bases from PAM) (+ strand)
gRNA:
5'-TTTTCAGTTTATATCAGCTTAGG-3'
ssODN:
5'-TAAAAATGAGCAAACTTTTAAATTAGGATTCAAGCCAGAACGTTTTTCAGTTTATATCAGNTTAGGGGCTATGTGCATGTAAAACAGATTAGAAAAGTTTCATGGCGAAGTAACTGAAGAT-3'
Low
Target 2 (3 bases from PAM) (+ strand)
gRNA:
5'-TTTCAGTTTATATCAGCTTAGGG-3'
ssODN:
5'-TAAAAATGAGCAAACTTTTAAATTAGGATTCAAGCCAGAACGTTTTTCAGTTTATATCAGNTTAGGGGCTATGTGCATGTAAAACAGATTAGAAAAGTTTCATGGCGAAGTAACTGAAGAT-3'
Low
Target 3 (4 bases from PAM) (+ strand)
gRNA:
5'-TTCAGTTTATATCAGCTTAGGGG-3'
ssODN:
5'-TAAAAATGAGCAAACTTTTAAATTAGGATTCAAGCCAGAACGTTTTTCAGTTTATATCAGNTTAGGGGCTATGTGCATGTAAAACAGATTAGAAAAGTTTCATGGCGAAGTAACTGAAGAT-3'
Low