TUSCAN

Uses

TUSCAN can help you find the most effictive CRISPR/Cas9 targets in a genomic region by ranking targets by the predicted cutting efficiency.

This prediction is based on genetic features such as the target sequence and nearby nucleotides

Results

TUSCAN finds every candidate target in your region of interest and for each candidate target, reports the TUSCAN score. This score represents the predicted cutting efficiency of CRISPR/Cas9 at that target, where a higher value is a higher predicted activity.