TUSCAN

Contents:


URL

https://0hbx4gdq2g.execute-api.ap-southeast-2.amazonaws.com/prod

Search by region (POST)

Searches for and scores CRISPR targets within the specified organism and location. Alternatively, just specify a sequence.

/submit

Parameters

FieldTypeDescription
speciesStringThe species to query (i.e. "homo_sapiens", "mus_musculus")
chromosomeStringThe chromosome to query (i.e. "1", "2", "X", "Y", "M")
startNumberThe start position of the sequence in the chromosome (1-based).
endNumberThe end position of the sequence in the chromosome (1-based).
FieldTypeDescription
sequenceStringThe nucleotide sequence. Should only contain the DNA characters [ATCGN].

Success 200

FieldTypeDescription
JobIDStringThe new Job-ID.

Error 400

FieldTypeDescription
messageStringA descriptive error message.

Retrieve job (GET)

Reads the job information

/results/:id

Parameters

FieldTypeDescription
idStringThe Job-ID

Success 200

FieldTypeDescription
idStringThe Job-ID.
genomeNumberThe reference genome name.
chromosomeStringThe chromosome name.
locationNumberThe location of the sequence in the chromosome.

Retrieve job targets (GET)

Reads the targets for a specific job

/results/:id/targets

Parameters

FieldTypeDescription
idStringThe Job-ID

Success 200

FieldTypeDescription
recordsTotalNumberThe total number of items matching the query.
dataArrayAn array of objects, each representing a target.
positionNumberThe position of the target in the input sequence.
locationNumberThe location of the target in the chromosome.
strandStringThe strand [+, -] the target is present on.
sequenceStringThe nucleotide sequence of the target.
gcNumberThe GC content of the target.
tuscanNumberThe TUSCAN score.
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